Application to optimize multiple enzyme parameters: The application was applied to improve an enzyme in the presence of different mediators across a range of pH values. The optimal combination of mutations was determined, leading to a mutant with 12-fold improvement in specificity for one mediator and 8-fold improvement in specificity for another mediator, compared to the wild-type enzyme, and better performance in three pH-adjusted buffers.
protein-team@peaccel.com
One-shot multi-parameter optimization of an enzyme
![](https://www.peaccel.com/wp-content/uploads/2021/11/One_shot_multi_parameter_optimization_of_an_enzyme.png)