One-shot multi-parameter optimization of an enzyme

Application to optimize multiple enzyme parameters: The application was applied to improve an enzyme in the presence of different mediators across a range of pH values. The optimal combination of mutations was determined, leading to a mutant with 12-fold improvement in specificity for one mediator and 8-fold improvement in specificity for another mediator, compared to the wild-type enzyme, and better performance in three pH-adjusted buffers.